Antibody conjugated metal nanoparticle decorated graphene sheets for a mycotoxin sensor. Issue 61 (13th June 2016)

• Record Type: Journal Article
• Authors: Srivastava, Saurabh; Kumar, Vinod; Arora, Kamal; Singh, Chandan; Ali, Md. Azahar; Puri, Nitin K.; Malhotra, Bansi D.
• Is Part Of: RSC advances. Volume 6:Issue 61(2016)
• Page Start: 56518
• Page End: 56526
• Publication Date: 2016-06-13
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c6ra04469g
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The proposed rGO–Ni NPs based immunosensor utilized for aflatoxin B1 detection indicates high sensitivity. Abstract : We demonstrate the fabrication of a label-free immunosensor for electrochemical detection of mycotoxin i.e. aflatoxin B1 (AFB1 ) using nickel nanoparticle decorated reduced graphene oxide (rGO–Ni NPs) sheets. The rGO–Ni NPs have been electrophoretically deposited onto an indium tin oxide (ITO) coated glass electrode. This fabricated immunosensor is sensitive to 0.16 ng mL −1 concentration of AFB1 in phosphate buffer solution. It exhibits a lower limit of detection when compared to the HPLC standard method (2.5 ng mL −1 ) and shows minimal interference with another food toxin (ochratoxin A). Electron microscopy, Fourier transform infrared, UV-visible spectroscopy and electrochemical techniques were utilized to confirm selective decoration of Ni NPs on the rGO sheets and to investigate the chemical and electrochemical properties of the nanocomposite. The highly crystalline properties of the rGO–Ni NPs sheets along with the excellent electro-catalytic properties of the Ni NPs result in a higher heterogeneous electron transfer rate indicating the enhanced sensitivity (129.6 μA ng −1 mL cm −2 ) of the device. The favorable environment for antibody conjugation on the surface of the rGO–Ni NPs sheets offers a low limit-of-detection (0.16 ng mL −1 ) for AFB1 .