Distribution of PSA-NCAM in normal, Alzheimer's and Parkinson's disease human brain. (25th August 2016)

• Record Type: Journal Article
• Authors: Murray, Helen C.; Low, Victoria F.; Swanson, Molly E.V.; Dieriks, Birger V.; Turner, Clinton; Faull, Richard L.M.; Curtis, Maurice A.
• Is Part Of: Neuroscience. Volume 330(2016)
• Page Start: 359
• Page End: 375
• Publication Date: 2016-08-25
• Subjects: AD Alzheimer's disease -- CA1 Cornu Ammonis area 1 -- CB cerebellum -- CN Caudate nucleus -- DAB 3, 3-diaminobenzidine chromogen -- DG dentate gyrus -- EC entorhinal cortex -- GAPDH glyceraldehyde-3-phosphate dehydrogenase -- GCL granule cell layer -- GFAP glial fibrillary acid protein -- HP hippocampus -- IOD integrated optical density -- MTG middle temporal gyrus -- NCAM neural cell adhesion molecule -- PCNA proliferating cell nuclear antigen -- PD Parkinson's disease -- PSA polysialic acid -- SFG superior frontal gyrus -- SGZ sub-granular zone -- SM sensory-motor cortex -- SN substantia nigra -- SVZ sub-ventricular zone -- VC visual cortex; polysialic acid -- neural cell adhesion molecule -- plasticity -- Alzheimer's disease -- tau -- Parkinson's disease; Neurochemistry -- Periodicals; Neurophysiology -- Periodicals; Neurology -- Periodicals; Neurochimie -- Périodiques; Neurophysiologie -- Périodiques; Neurochemistry; Neurophysiology; Electronic journals; Periodicals; Electronic journals; 612.8
• DOI: 10.1016/j.neuroscience.2016.06.003
• Languages: English
• ISSNs: 0306-4522

Abstract

Highlights: PSA-NCAM expression in the human brain is widespread, yet specific. PSA-NCAM cells are detected in the matrix compartment of the caudate nucleus. PSA-NCAM cells are detected throughout the cerebellum. Many PSA-NCAM cells express mature neuronal markers such as NeuN. Reduced PSA-NCAM in the Alzheimer's disease entorhinal cortex correlates with tau load. Abstract: Polysialated neural cell adhesion molecule (PSA-NCAM) is a membrane bound glycoprotein widely expressed during nervous system development. While commonly described in the neurogenic niches of the adult human brain, there is limited evidence of its distribution in other brain regions. PSA-NCAM is an important regulator of cell–cell interactions and facilitates cell migration and plasticity. Recent evidence suggests these functions may be altered in neurodegenerative diseases such as Alzheimer's (AD) and Parkinson's disease (PD). This study provides a detailed description of the PSA-NCAM distribution throughout the human brain and quantitatively compares the staining load in cortical regions and sub-cortical structures between the control, AD and PD brain. Our results provide evidence of widespread, yet specific, PSA-NCAM expression throughout the human brain including regions devoid of PSA-NCAM in the rodent brain such as the caudate nucleus (CN) and cerebellum (CB). We also detected a significant reduction in PSA-NCAM load in the entorhinal cortex (EC) of cases that was inversely correlated with hyperphosphorylated tau load. These results demonstrate that PSA-NCAM-mediated structural plasticity may not be limited to neurogenic niches and is conserved in the aged brain. We also provide evidence that PSA-NCAM is reduced in the EC, a region severely affected by AD pathology.