Dynamic Histone Acetylation of H3K4me3 Nucleosome Regulates MCL1 Pre‐mRNA Splicing. Issue 10 (6th March 2016)

• Record Type: Journal Article
• Authors: Khan, Dilshad H.; Gonzalez, Carolina; Tailor, Nikesh; Hamedani, Mohammad K.; Leygue, Etienne; Davie, James R.
• Is Part Of: Journal of cellular physiology. Volume 231:Issue 10(2016:Oct.)
• Page Start: 2196
• Page End: 2204
• Publication Date: 2016-03-06
• Subjects: Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.25337
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : Pre‐mRNA splicing is a cotranscriptional process affected by the chromatin architecture along the body of coding genes. Recruited to the pre‐mRNA by splicing factors, histone deacetylases (HDACs) and K‐acetyltransferases (KATs) catalyze dynamic histone acetylation along the gene. In colon carcinoma HCT 116 cells, HDAC inhibition specifically increased KAT2B occupancy as well as H3 and H4 acetylation of the H3K4 trimethylated (H3K4me3) nucleosome positioned over alternative exon 2 of the MCL1 gene, an event paralleled with the exclusion of exon 2. These results were reproduced in MDA‐MB‐231, but not in MCF7 breast adenocarcinoma cells. These later cells have much higher levels of demethylase KDM5B than either HCT 116 or MDA‐MB‐231 cells. We show that H3K4me3 steady‐state levels and H3K4me3 occupancy at the end of exon 1 and over exon 2 of the MCL1 gene were lower in MCF7 than in MDA‐MB‐231 cells. Furthermore, in MCF7 cells, there was minimal effect of HDAC inhibition on H3/H4 acetylation and H3K4me3 levels along the MCL1 gene and no change in pre‐mRNA splicing choice. These results show that, upon HDAC inhibition, the H3K4me3 mark plays a critical role in the exclusion of exon 2 from the MCL1 pre‐mRNA. J. Cell. Physiol. 231: 2196–2204, 2016. © 2016 Wiley Periodicals, Inc.