Β-naphthoflavone interferes with cyp1c1, cox2 and IL-8 gene transcription and leukotriene B4 secretion in Atlantic cod (Gadus morhua) head kidney cells during inflammation. (July 2016)

• Record Type: Journal Article
• Authors: Holen, Elisabeth; Olsvik, Pål A.
• Is Part Of: Fish & shellfish immunology. Volume 54(2016:Jul.)
• Page Start: 128
• Page End: 134
• Publication Date: 2016-07
• Subjects: β -naphthoflavone -- Cod head kidney cells -- LPS -- cyp1c1 -- cox2, IL-8 -- LTB4; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2016.03.043
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: The objective of this study was to evaluate how β-naphthoflavone interacts with lipopolysaccharide (LPS) and polyinosinic acid: polycytidylic acid (poly I: C) induced innate immune parameters as well as phase I and phase II detoxification enzymes in head kidney cells isolated from Atlantic cod. β-naphthoflavone is a pure agonist of aryl hydrocarbon receptor (AhR) while LPS and poly I: C are not. β-naphthoflavone was added to head kidney leukocytes alone or together with LPS or poly I: C and the responses were evaluated in terms of protein and gene expression. The results showed that β-naphthoflavone (25 nM), with and without LPS, significantly induced cytochrome P450 ( cyp1c ) transcription in cod head kidney cells. β-naphthoflavone (100 nM) in the presence of the virus mimic, poly I: C, also increased cyp1c1 transcription. LPS induced cyp1c1, cyclooxygenase 2 ( cox2 ), interleukin 1β (IL-1β ), interleukin 6 ( IL-6 ) and interleukin 8 ( IL-8 ) transcription, genes that were not affected by the tested β-naphthoflavone concentrations alone. However, β-naphthoflavone (25 and 50 nM) strengthened LPS induced cox2 and IL-8 transcription. Cod head kidney cells exposed to β-naphthoflavone concentrations ranging from 25 to 100 nM, with and without LPS or poly I: C, expressed AhR protein. LPS or β-naphthoflavone (5–50 nM) significantly induced leukotriene B4 (LTB4 ) secretion compared to control. In conclusion, this study suggests that β-naphthoflavone could interfere with LPS induced immune cell signaling in cod head kidney cells. Highlights: β -naphthoflavone increased LPS-induced cox2 and IL-8 transcription. β -naphthoflavone (5–50 nM) increased LTB4 secretion. β -naphthoflavone induced AhR protein expression in cod head kidney cells.