Enhancement of poly-3-hydroxybutyrate production in Synechocystis sp. PCC 6803 by overexpression of its native biosynthetic genes. (August 2016)

• Record Type: Journal Article
• Authors: Khetkorn, Wanthanee; Incharoensakdi, Aran; Lindblad, Peter; Jantaro, Saowarath
• Is Part Of: Bioresource technology. Volume 214(2016)
• Page Start: 761
• Page End: 768
• Publication Date: 2016-08
• Subjects: pha overexpression -- Polyhydroxybutyrate -- Synechocystis sp. PCC 6803; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.05.014
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Native biosynthetic genes for PHB production overexpressed in Synechocystis. Overexpression of phaA and phaB resulted in the highest level of PHB. Metabolic engineering leads to increased production of the valuable polymer PHB. Abstract: Synechocystis sp. PCC 6803 strains overexpressing pha genes were constructed and characterized for poly-3-hydroxybutyrate (PHB) production. These pha overexpressing strains showed slightly reduced growth rates. Under N-deprived condition, the strains overexpressing (OE) phaAB, phaEC and phaABEC showed significantly higher PHB contents than the wild type. The maximum PHB content, a 2.6-fold increase producing 26% PHB (dcw), was observed in OE phaAB cells grown for 9 days in N-deprived medium. Under this condition, these OE phaAB cells increased PHB production to 35% PHB (dcw) upon addition of 0.4% (w/v) acetate. Higher PHB granules in OE phaAB cells were clearly visualized by both Nile red staining and TEM imaging. All OE strains under N-deficient condition had increased glgX transcript levels. Overall results demonstrate an enhanced PHB production in Synechocystis cells overexpressing pha genes, particularly phaA and phaB, when grown in N-deprived medium containing 0.4% (w/v) acetate.