Hybridization-based aptamer labeling using complementary oligonucleotide platform for PET and optical imaging. (September 2016)

• Record Type: Journal Article
• Authors: Park, Jun Young; Lee, Tae Sup; Song, In Ho; Cho, Ye Lim; Chae, Ju Ri; Yun, Mijin; Kang, Hyungu; Lee, Jung Hwan; Lim, Jong Hoon; Cho, Won Gil; Kang, Won Jun
• Is Part Of: Biomaterials. Volume 100(2016)
• Page Start: 143
• Page End: 151
• Publication Date: 2016-09
• Subjects: Aptamer -- Molecular imaging -- Hybridization -- Oligonucleotide -- Radioisotope -- PET; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2016.05.035
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Aptamers are promising next-generation ligands used in molecular imaging and theragnosis. Aptamers are synthetic nucleic acids that can be held together with complementary sequences by base-pair hybridization. In this study, the complementary oligonucleotide (cODN) hybridization-based aptamer conjugation platform was developed to use aptamers as the molecular imaging agent. The cODN was pre-labeled with fluorescent dye or radioisotope and hybridized with a matched sequence containing aptamers in aqueous conditions. The cODN platform-hybridized aptamers exhibited good serum stability and specific binding affinity towards target cancer cells both in vitro and in vivo . These results suggest that the newly designed aptamer conjugation platform offers great potential for the versatile application of aptamers as molecular imaging agents.