An association between neuropeptide Y levels and leukocyte subsets in stress-exacerbated asthmatic mice. (June 2016)

• Record Type: Journal Article
• Authors: Lu, Yanxia; Ho, Roger Chun-Man
• Is Part Of: Neuropeptides. Volume 57(2016)
• Page Start: 53
• Page End: 58
• Publication Date: 2016-06
• Subjects: Stress -- Neuropeptide Y -- Asthma -- Cell infiltration -- Airway inflammation; Neuropeptides -- Periodicals; Neuropeptides; Neuropeptides -- Périodiques; Neuropeptides; Electronic journals; Periodicals; 572.65
• DOI: 10.1016/j.npep.2015.11.091
• Languages: English
• ISSNs: 0143-4179

Abstract

Abstract: Neuropeptide Y (NPY) was recently proposed to be associated with stress and airway inflammation; however, this has rarely been studied in animal models of asthma. Twenty-four C57BL/6 mice were randomly divided into 3 groups of 8 each: naive control group, asthma group (with an established asthma model), and stressed asthma group (with established asthma and stress models). Bronchoalveolar lavage (BAL) fluid was collected for total cell counts using a hemocytometer and for cytological examinations by Wright stain. Differential inflammatory cell counts were performed to identify eosinophils, macrophages, neutrophils, and lymphocytes. NPY and corticosterone serum levels were determined with enzyme immunoassay kits. Stress was associated with increased airway inflammatory response, which was manifested by the accumulation of total leukocytes and eosinophils in the BAL fluid in comparison with the asthma and the control groups. The levels of NPY ( p < 0.05) and corticosterone ( p < 0.01) were elevated in the stressed asthma group in comparison with the control and asthma groups. The concentration of NPY and corticosterone positively correlated with the total leukocyte count ( r = 0.892, p < 0.05 and r = 0.937, p < 0.01 respectively) and eosinophil numbers ( r = 0.806, p = 0.053 and r = 0.885, p < 0.01 respectively). Stress may be associated with elevated peripheral NPY level, which was observed to be associated with exacerbated airway inflammation in asthmatic mice. Highlights: The role of NPY in the stress–asthma link has rarely been studied in animal models. Mice with stress-aggravated asthma displayed elevated serum NPY levels. Higher NPY after stress is related to accumulation of leukocytes and eosinophils.