Akirin interacts with Bap60 and 14-3-3 proteins to regulate the expression of antimicrobial peptides in the kuruma shrimp (Marsupenaeus japonicus). (February 2016)

• Record Type: Journal Article
• Authors: Liu, Ning; Wang, Xian-Wei; Sun, Jie-Jie; Wang, Lei; Zhang, Hong-Wei; Zhao, Xiao-Fan; Wang, Jin-Xing
• Is Part Of: Developmental and comparative immunology. Volume 55(2016)
• Page Start: 80
• Page End: 89
• Publication Date: 2016-02
• Subjects: Akirin -- NF-κB -- Relish -- Bap60 -- 14-3-3; Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.10.015
• Languages: English
• ISSNs: 0145-305X

Abstract

Abstract: Akirin is a recently discovered nuclear factor that plays important roles in innate immune responses. Akirin is a positive regulator of the NF-κB factor of the Drosophila immune deficiency (IMD) pathway, which shares extensive similarities with the mammalian tumor necrosis factor receptor (TNFR) signaling pathway. However, some studies found that the NF-κB transcriptional targets were also strongly repressed in akirin2 knockout mice following TLR, IL-1β and TNFα treatment. Therefore, the function of Akirin in the immune response requires further clarification. In this study, an Akirin homolog in the kuruma shrimp ( Marsupenaeus japonicus ) was identified. It was mainly expressed in hemocytes, heart and intestines. The expression of Akirin was upregulated by challenge with the Gram-negative bacterium Vibrio anguillarum, but was not significantly influenced by challenge with the Gram-positive bacterium Staphylococcus aureus . Knockdown of Akirin suppressed the expression of several IMD-Relish target effectors (antimicrobial peptides, AMPs). The limited regulating spectrum of Akirin might be associated with Bap60, a component of the Brahma (SWI/SNF) ATP-dependent chromatin-remodeling complex. In addition, Akirin also interacts with 14-3-3, which inhibited the expression of Akirin-target AMPs. The results suggested that Akirin is involved in the IMD-Relish pathway by interacting with Relish. The interaction of Akirin with Bap60 positively regulated the Akirin–Relish function, and its interaction with 14-3-3 negatively regulated the Akirin–Relish function. Highlights: Shrimp Akirin is involved in the IMD-Relish pathway by interacting with Relish. Interaction of Akirin with Bap60 positively regulated the Akirin–Relish function. Interaction of Akirin with 14-3-3 negatively regulated the Akirin–Relish function.