Conversion of Prostate Adenocarcinoma to Small Cell Carcinoma‐Like by Reprogramming. Issue 9 (4th February 2016)

• Record Type: Journal Article
• Authors: Borges, Gisely T.; Vêncio, Eneida F.; Quek, Sue‐Ing; Chen, Adeline; Salvanha, Diego M.; Vêncio, Ricardo Z.N.; Nguyen, Holly M.; Vessella, Robert L.; Cavanaugh, Christopher; Ware, Carol B.; Troisch, Pamela; Liu, Alvin Y.
• Is Part Of: Journal of cellular physiology. Volume 231:Issue 9(2016:Sep.)
• Page Start: 2040
• Page End: 2047
• Publication Date: 2016-02-04
• Subjects: Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.25313
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : The lineage relationship between prostate adenocarcinoma and small cell carcinoma was studied by using the LuCaP family of xenografts established from primary neoplasm to metastasis. Expression of four stem cell transcription factor (TF) genes, LIN28A, NANOG, POU5F1, SOX2, were analyzed in the LuCaP lines. These genes, when force expressed in differentiated cells, can reprogram the recipients into stem‐like induced pluripotent stem (iPS) cells. Most LuCaP lines expressed POU5F1, while LuCaP 145.1, representative of small cell carcinoma, expressed all four. Through transcriptome database query, many small cell carcinoma genes were also found in stem cells. To test the hypothesis that prostate cancer progression from "differentiated" adenocarcinoma to "undifferentiated" small cell carcinoma could involve re‐expression of stem cell genes, the four TF genes were transduced via lentiviral vectors into five adenocarcinoma LuCaP lines—70CR, 73CR, 86.2, 92, 105CR—as done in iPS cell reprogramming. The resultant cells from these five transductions displayed a morphology of small size and dark appearing unlike the parentals. Transcriptome analysis of LuCaP 70CR* ("*" to denote transfected progeny) revealed a unique gene expression close to that of LuCaP 145.1. In a prostate principal components analysis space based on cell‐type transcriptomes, the different LuCaP transcriptome datapoints were aligned to suggest a possible ordered sequence of expression changes from the differentiated luminal‐like adenocarcinoma cell types to the less differentiated, more stem‐like small cell carcinoma types, and LuCaP 70CR*. Prostate cancer progression can thus be molecularly characterized by loss of differentiation with re‐expression of stem cell genes. J. Cell. Physiol. 231: 2040–2047, 2016. © 2016 Wiley Periodicals, Inc. Abstract : Stem cell genes are re‐expressed in prostate cancer progression. Differentiated prostate adenocarcinoma could be converted to undifferentiated small cell carcinoma‐like by reprogramming.