Photolytic Protein Aggregates: Versatile Materials for Controlled Release of Active Proteins. Issue 9 (4th March 2016)

• Record Type: Journal Article
• Authors: Ishiwatari, Akira; Yamaguchi, Satoshi; Takamori, Satoshi; Yamahira, Shinya; Minamihata, Kosuke; Nagamune, Teruyuki
• Is Part Of: Advanced healthcare materials. Volume 5:Issue 9(2016)
• Page Start: 1002
• Page End: 1007
• Publication Date: 2016-03-04
• Subjects: biomaterials;·drug delivery -- mammalian cells -- photolysis -- protein engineering; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/adhm.201500957
• Languages: English
• ISSNs: 2192-2640

Abstract

Abstract : Photolytic protein aggregates are developed as a facile and versatile platform for light‐induced release of active proteins. The proteins modified with biotin through a photo‐cleavable linker rapidly form aggregates with streptavidin and biotinylated functional molecules simply by mixing. Light irradiation releases active proteins from the aggregates in high yields, and light‐induced uptake of drug‐modified transferrin into living cells is successfully demonstrated.