Green tea (-)-epigallocatechin gallate induced growth inhibition of human placental choriocarcinoma cells. (May 2016)

• Record Type: Journal Article
• Authors: Shih, Li-Jane; Lin, Yu-Ren; Lin, Cheng-Kuo; Liu, Hang-Shen; Kao, Yung-Hsi
• Is Part Of: Placenta. Volume 41(2016:May)
• Page Start: 1
• Page End: 9
• Publication Date: 2016-05
• Subjects: Green tea -- Epigallocatechin gallate -- Placenta -- Choriocarcinoma cell -- Mitogen-activated protein kinase -- AMP-activated protein kinase; Placenta -- Periodicals; Reproduction -- Periodicals; Placenta -- Periodicals; Placenta -- Périodiques; Reproduction -- Périodiques; 612.63
• DOI: 10.1016/j.placenta.2016.02.017
• Languages: English
• ISSNs: 0143-4004

Abstract

Abstract: This study investigated the pathways involved in the effect of green tea epigallocatechin gallate (EGCG) on mitogenesis in BeWo, JEG-3, and JAR placental choriocarcinoma cells. EGCG inhibited cell proliferation in dose-dependent and time-dependent manners, as indicated by the number of cells and incorporation of bromodeoxyuridine (BrdU). A catechin-specific effect of green tea was evident; EGCG was more effective than epicatechin, epicatechin gallate, and epigallocatechin in suppressing cell growth. When all three of the mitogen-activated protein kinase (MAPK) subfamilies, i.e., ERK, p38, and JNK, were examined, EGCG significantly increased levels of phospho-ERK1/2 (pERK1/2) and phospho-p38 (pp38) and did not alter the total protein levels of ERK1/2, p38 MAPK, JNK, and phospho-JNK. EGCG-induced increases in the levels of pERK1/2 and pp38 proteins were prevented by pre-treatment with specific inhibitors of ERK1/2 MAPK and p38 MAPK, respectively. These inhibitors also suppressed EGCG-induced decreases in both cell number and BrdU incorporation. Moreover, pre-treatment with an AMP-activated protein kinase (AMPK) inhibitor prevented the actions of EGCG on proliferation and AMPK phosphorylation. These data suggest that EGCG mediates choriocarcinoma cell growth via the AMPK, ERK, and p38 pathways, but not JNK pathway. Highlights: EGCG induced growth inhibition of human placental choriocarcinoma cells. A catechin-specific effect of green tea was evident by the greatest effect of EGCG. The EGCG signal in cancerous trophoblast mitogenesis depends on the ERK, p38 and AMPK pathways.