Scallop larvae hatcheries as source of bacteria carrying genes encoding for non-enzymatic phenicol resistance. Issue 1 (15th June 2015)

• Record Type: Journal Article
• Authors: Miranda, Claudio D.; Rojas, Rodrigo; Geisse, Julieta; Romero, Jaime; González-Rocha, Gerardo
• Is Part Of: Marine pollution bulletin. Volume 95:Issue 1(2015:Jun.)
• Page Start: 173
• Page End: 182
• Publication Date: 2015-06-15
• Subjects: Florfenicol -- Antimicrobial resistance -- floR -- Scallop hatchery -- Chile; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2015.04.026
• Languages: English
• ISSNs: 0025-326X

Abstract

Highlights: Most of florfenicol-resistant isolates from scallop hatcheries carried the floR gene. Most of bacteria from untreated and treated scallop larvae harbored the floR gene. Most of the floR -carrying strains belonged to Pseudomonas and Pseudoalteromonas genus. High co-resistance to oxytetracycline and streptomycin of floR -carrying strains. Scallop larvae and hatchery effluents are important sources of floR -carrying bacteria. Abstract: The main aim of the study was to evaluate the role of scallop hatcheries as source of the floR and cmlA genes. A number of 133 and 121 florfenicol-resistant strains were isolated from scallop larval cultures prior to their transfer to seawater and from effluent samples from 2 commercial hatcheries and identified by 16S rRNA gene sequence analysis, observing a predominance of the Pseudomonas, Pseudoalteromonas and Halomonas genera and exhibiting an important incidence of co-resistance to streptomycin, oxytetracycline and co-trimoxazole. A high percentage of strains from both hatcheries carried the floR gene (68.4% and 89.3% of strains), whereas a lower carriage of the cmlA gene was detected (27.1% and 54.5% of strains). The high prevalence of floR -carrying bacteria in reared scallop larvae and hatchery effluents contributes to enrich the marine resistome in marine environments, prompting the need of a continuous surveillance of these genes in the mariculture environments.