In vitro mechanistic and in vivo anti-tumor studies of Glycosmis pentaphylla (Retz.) DC against breast cancer. (20th June 2016)

• Record Type: Journal Article
• Authors: Shoja, M.H.; Reddy, Neetinkumar D.; Nayak, Pawan G.; Biswas, Subhankar; Srinivasan, K.K.; Rao, C. Mallikarjuna
• Is Part Of: Journal of ethnopharmacology. Volume 186(2016)
• Page Start: 159
• Page End: 168
• Publication Date: 2016-06-20
• Subjects: DMEM Dulbecco's minimum essential media -- FBS fetal bovine serum -- HIF-1α hypoxia inducible factor-1 alpha -- MMP-9 matrix metalloproteinase-9 -- NP-40 Nonidet P-40 -- PBS Phosphate Buffer Saline -- OD Optical Density -- SDS sodium dodecyl sulfate -- OECD Organisation for Economic Co-Operation and Development -- CMC carboxymethyl cellulose -- p.o. per oral -- DMBA 7, 12-Dimethylbenz(a)anthracene -- S.E.M. standard error of mean; Glycosmis pentaphylla (Retz.) DC -- Breast cancer -- Apoptosis -- Western blot -- Metastasis -- Angiogenesis; Ethnopharmacology -- Periodicals; Pharmacognosy -- Periodicals; Herbs -- Periodicals; Herbs -- Periodicals; Pharmacognosy -- Periodicals; Pharmacognosie -- Périodiques; Herbes -- Périodiques; 615.1
• DOI: 10.1016/j.jep.2016.04.002
• Languages: English
• ISSNs: 0378-8741

Abstract

Abstract: Ethnopharmacological relevance: Glycosmis pentaphylla (Retz.) DC (Rutaceae) has been traditionally used for the treatment of rheumatism, cancer, liver disorders, inflammation etc. Aim of the study: The present study is aimed at elucidating the effect of Glycosmis pentaphylla (Retz.) DC on the key markers of apoptosis, metastasis and angiogenesis, in vitro . The study also evaluated the effect of fractions in vivo in DMBA-induced mammary tumor model. Materials and methods: Fractions of Glycosmis pentaphylla (Retz.) DC leaf extracts was studied for their effect on apoptotic markers in breast cancer cell lines, MCF-7 and MDA-MB-231 cells. They were also studied for their effect on metastatic and angiogenic markers, MMP-9 and HIF-1α in MCF-7 cells. The fractions were studied in vivo in DMBA-induced mammary tumor model in Sprague Dawley rats. Results: The studies showed that the fractions induced apoptosis in breast cancer cells through the intrinsic/mitochondrial apoptotic pathway. The fractions were also able to inhibit the metastatic and angiogenic markers, MMP-9 and HIF-1α. Anti-tumor studies in DMBA-induced mammary model in Sprague Dawley rats also showed favorable results. Graphical abstract: