Cost-effective and sensitive colorimetric immunosensing using an iron oxide-to-Prussian blue nanoparticle conversion strategy. Issue 12 (3rd May 2016)
- Record Type:
- Journal Article
- Title:
- Cost-effective and sensitive colorimetric immunosensing using an iron oxide-to-Prussian blue nanoparticle conversion strategy. Issue 12 (3rd May 2016)
- Main Title:
- Cost-effective and sensitive colorimetric immunosensing using an iron oxide-to-Prussian blue nanoparticle conversion strategy
- Authors:
- Fu, Guanglei
Sanjay, Sharma T.
Li, XiuJun - Abstract:
- Abstract : A novel iron oxide-to-Prussian blue nanoparticle conversion strategy has been developed and applied to a sensitive, cost-effective and user-friendly colorimetric immunoassay without the aid of any complex signal amplification processes. Abstract : The development of new sensitive, cost-effective and user-friendly colorimetric bioassays is in increasing demand to meet the requirement of modern clinical diagnostics and field detection. Herein, a novel iron oxide-to-Prussian blue (PB) nanoparticle (NP) conversion strategy was developed and applied to sensitive colorimetric immunosensing of cancer biomarkers. In a typical sandwich-type immunosensing system, the captured spherical antibody-conjugated iron oxide NPs were transformed into cubic PB NPs, which exhibited a highly visible blue color with high molar extinction coefficients. Hence, a new colorimetric immunosensing strategy was developed as a result of this low cost and simple transformation process. Without the aid of any complex nanoparticle stabilizing ligands and signal amplification processes, prostate-specific antigen as a model analyte can be detected at a concentration as low as 1.0 ng mL −1 by the naked eye with good reliability for detection of real human serum samples. This is the first attempt to develop and apply the iron oxide-to-PB NP colorimetric conversion strategy for immunosensing, and shows great promise for the development of new sensitive, cost-effective and user-friendly colorimetricAbstract : A novel iron oxide-to-Prussian blue nanoparticle conversion strategy has been developed and applied to a sensitive, cost-effective and user-friendly colorimetric immunoassay without the aid of any complex signal amplification processes. Abstract : The development of new sensitive, cost-effective and user-friendly colorimetric bioassays is in increasing demand to meet the requirement of modern clinical diagnostics and field detection. Herein, a novel iron oxide-to-Prussian blue (PB) nanoparticle (NP) conversion strategy was developed and applied to sensitive colorimetric immunosensing of cancer biomarkers. In a typical sandwich-type immunosensing system, the captured spherical antibody-conjugated iron oxide NPs were transformed into cubic PB NPs, which exhibited a highly visible blue color with high molar extinction coefficients. Hence, a new colorimetric immunosensing strategy was developed as a result of this low cost and simple transformation process. Without the aid of any complex nanoparticle stabilizing ligands and signal amplification processes, prostate-specific antigen as a model analyte can be detected at a concentration as low as 1.0 ng mL −1 by the naked eye with good reliability for detection of real human serum samples. This is the first attempt to develop and apply the iron oxide-to-PB NP colorimetric conversion strategy for immunosensing, and shows great promise for the development of new sensitive, cost-effective and user-friendly colorimetric bioassays in various bioanalytical applications, especially in low-resource settings. … (more)
- Is Part Of:
- Analyst. Volume 141:Issue 12(2016)
- Journal:
- Analyst
- Issue:
- Volume 141:Issue 12(2016)
- Issue Display:
- Volume 141, Issue 12 (2016)
- Year:
- 2016
- Volume:
- 141
- Issue:
- 12
- Issue Sort Value:
- 2016-0141-0012-0000
- Page Start:
- 3883
- Page End:
- 3889
- Publication Date:
- 2016-05-03
- Subjects:
- Chemistry, Analytic -- Periodicals
543 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/an?e=1#!issueid=an139020&type=current&issnprint=0003-2654 ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c6an00254d ↗
- Languages:
- English
- ISSNs:
- 0003-2654
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 0893.000000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 355.xml