Effect of a new recombinant Aeromonas hydrophila vaccine on the grass carp intestinal microbiota and correlations with immunological responses. Issue 1 (July 2015)

• Record Type: Journal Article
• Authors: Liu, Lei; Gong, Yu-Xin; Zhu, Bin; Liu, Guang-Lu; Wang, Gao-Xue; Ling, Fei
• Is Part Of: Fish & shellfish immunology. Volume 45:Issue 1(2015:Jul.)
• Page Start: 175
• Page End: 183
• Publication Date: 2015-07
• Subjects: Intestine microbiome -- Aeromonas hydrophila -- SWCNT vaccine -- Bath immunization -- Immune response; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.03.043
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Intestinal microbiota has become an integral component of the fish, and plays a key role in host metabolism, immunity and health maintenance. However, information on the immune responses after vaccine administration in relation to the intestinal microbiota is absent in fish. The present study focused on the effect of a new recombinant Aeromonas hydrophila vaccine (Aera) by using a novel functionalized, single-walled carbon nanotubes (SWCNTs) as a delivery vehicle on the intestinal microbiota of grass carp ( Ctenopharyngodon idella ) through the bath immunization, and further explored the immunological responses in intestine, kidney and spleen. By performing deep sequencing, a total of 81, 979 valid reads and 609 OTUs obtained from 4 intestine samples were analyzed. We detected 141 genuses, most of which belonged to Firmicutes, Fusobacteria and Proteobacteria. Of note, the quantity of Aeromonas in library Aera (after 6 h Aera vaccine pretreatment, fish were transferred to tanks without SWCNTs-Aera for 28 d) and Aera-GD (6 h Aera vaccine pretreated prior to the group injected by A. hydrophila ) was declined 6.5% and 14.6% compared with the control, respectively. Moreover, the expression of seven immune-related genes (IFN-I, TNF-α, CRP, IL-8, IgM, MHC I and CD8α) in the intestine, kidney and spleen of Aera treated fish was significantly enhanced, which indicated that a better tissue immune response in grass carp was induced by the SWCNTs-Aera vaccine. Therefore, a new recombinant SWCNT-Aera vaccine may represent potentially efficient and immunological role in grass carp intestine to resist A. hydrophila infection. Highlights: The SWCNT-Aera vaccine represents a specific role in grass carp gut to resist Aeromonas hydrophila. The bath immunization of Aera could not lead to alterations of the major microbiota. The Aera vaccine produces a direct opposed response to Aeromonas burden in the intestine. A better tissue immune response in grass carp immunized with the Aera was induced.