Microbial decomposition of 13C- labeled phytosiderophores in the rhizosphere of wheat: Mineralization dynamics and key microbial groups involved. (July 2016)

• Record Type: Journal Article
• Authors: Oburger, Eva; Gruber, Barbara; Wanek, Wolfgang; Watzinger, Andrea; Stanetty, Christian; Schindlegger, Yvonne; Hann, Stephan; Schenkeveld, Walter D.C.; Kraemer, Stephan M.; Puschenreiter, Markus
• Is Part Of: Soil biology and biochemistry. Volume 98(2016)
• Page Start: 196
• Page End: 207
• Publication Date: 2016-07
• Subjects: 2′-deoxymugineic acid -- Microbial turnover -- Half-life -- Triticum aestivum cv tamaro -- Microbial activity -- PLFA-SIP -- Sorption -- Root exudates; Phytosiderophores (PS) 2′-deoxymugineic acid (DMA) -- water content (WC) diethylene triamine pentaacetic acid (DTPA) phospholipid fatty acid (PLFA) -- stable isotope probing (SIP) low molecular weight carbon (LMW-C); Soil biochemistry -- Periodicals; Soil biology -- Periodicals; Sols -- Biochimie -- Périodiques; Sols -- Biologie -- Périodiques; Sols -- Microbiologie -- Périodiques; Bodembiologie; Biochemie; 631.46
• DOI: 10.1016/j.soilbio.2016.04.014
• Languages: English
• ISSNs: 0038-0717

Abstract

Abstract: Being low molecular weight carbon (LMW-C) compounds, phytosiderophores (PS) released by strategy II plants are highly susceptible to microbial decomposition. However, to date very little is known about the fate of PS in soil. Using in-house synthesized 13 C4 -2′-deoxymugineic acid (DMA), the main PS released by wheat, we investigated DMA mineralization dynamics, including microbial incorporation into phospholipid fatty acids (PLFA), in the wheat rhizosphere and bulk soil of two alkaline and one acidic soil. Half-lives of the intact DMA molecule (3–8 h) as well as of DMA-derived C-compounds (8–38 days) were in the same order of magnitude as those published for other LMW-C compounds like sugars, amino acids and organic acids. Combining mineralization with PLFA data showed that between 40 and 65% of the added DMA was either respired or incorporated into soil microbial biomass after 24 h, with the largest part of total incorporated DMA- 13 C being recovered in gram negative bacteria. Considering root growth dynamics and that PS are mainly exuded from root tips, the significantly slower mineralization of DMA in bulk soil is of high ecological importance to enhance the Fe scavenging efficiency of PS released into the soil. Highlights: Half-lives of 13 C-DMA were in the same range as other LMW root exudate compounds. Gram-negative bacteria were mainly responsible for 13 C-MA decomposition. 13 C-DMA decomposition in the bulk soil was slower than in the rhizosphere. Sorption processes buffer initial microbial uptake from the soil solution.