Complexes of star-shaped cationic polyelectrolytes with anionic liposomes: Towards multi-liposomal assemblies with controllable stability. (14th June 2016)

• Record Type: Journal Article
• Authors: Sybachin, Andrey V.; Zaborova, Olga V.; Pergushov, Dmitry V.; Zezin, Alexander B.; Plamper, Felix A.; Müller, Axel H.E.; Kesselman, Ellina; Schmidt, Judith; Talmon, Yeshayahu; Menger, Fredric M.; Yaroslavov, Alexander A.
• Is Part Of: Polymer. Volume 93(2016)
• Page Start: 198
• Page End: 203
• Publication Date: 2016-06-14
• Subjects: Liposomes -- Self-assembly -- Nanocontainers -- Star-shaped polycation; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; 547.7
• DOI: 10.1016/j.polymer.2016.04.025
• Languages: English
• ISSNs: 0032-3861

Abstract

Abstract: Complexes were formed via the electrostatic interaction between 30–50 nm anionic liposomes and a star-shaped polyelectrolyte, poly{[2-(methacryloyloxy)ethyl]trimethyl ammonium iodide}, having cationic arms that radiate from a silicon-based central core. The complexation was investigated with attention given to assessing the capacity of the cationic stars for the anionic liposomes (both liquid and solid); the integrity of the complexed liposomes; and the stability of the resulting star/liposome complexes in aqueous salt solutions. We have found that by changing the content of anionic groups in the liposomal membrane as well as the phase-state of membrane, the stability of star/liposome complexes in aqueous salt media can be controlled. The liquid liposomes with 0.1 mol fraction of anionic palmitoyloleoylphosphatidylserine (POPS 1− ), and solid liposomes with 0.1 mol fraction of POPS 1−, retain their integrity when bound to the stars, with the resulting star/liposome complexes being stable in physiological solution, i.e. [NaCl] = 0.15 M. Multi-liposomal complexes containing up to 12 liposomes per star seem to hold promise as carriers for biologically active compounds. Graphical abstract: Highlights: Multiliposomal complexes of the liposomes of various composition assembled on polycationic stars are described. The requirements for the star/liposomes complexes with intact vesicles, remaining stable in physiological solution, are formulated. Liposome/star complexes are visualized by using cryo-TEM.