Cloning and expression of trehalose‐6‐phosphate synthase 1 from Rhizopus oryzae. (16th November 2015)

• Record Type: Journal Article
• Authors: Ozer Uyar, Ebru; Yücel, Meral; Hamamcı, Haluk
• Is Part Of: Journal of basic microbiology. Volume 56:issue 5(2016:May)
• Page Start: 459
• Page End: 468
• Publication Date: 2015-11-16
• Subjects: Cloning -- Rhizopus oryzae -- Trehalose -- Trehalose‐6‐phosphate synthase; Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201500425
• Languages: English
• ISSNs: 0233-111X

Abstract

Abstract : Trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. In fungi, Trehalose‐6‐phosphate synthase 1 (TPS1) plays a key role in the biosynthesis of trehalose. In this study, a full‐length cDNA from Rhizopus oryzae encoding TPS1 (designated as RoTPS1 ) was isolated. The RoTPS1 cDNA is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8 kDa. The amino acid sequence of RoTPS1 has a relatively high homology with the TPS1s in several other filamentous fungi. RoTPS1 was cloned into Saccharomyces cerevisiae and secretively expressed.