Bioassay-guided separation and identification of anticancer compounds in Tagetes erecta L. flowers. Issue 15 (7th April 2016)

• Record Type: Journal Article
• Authors: Lu, Hong; Yang, Shanshan; Ma, Hong; Han, Zhao; Zhang, Yaozhou
• Is Part Of: Analytical methods. Volume 8:Issue 15(2016)
• Page Start: 3255
• Page End: 3262
• Publication Date: 2016-04-07
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c5ay03256c
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Investigating bioactive components in marigold ( Tagetes erecta L.) can provide worthy information for drug development. Abstract : Investigating bioactive components in marigold ( Tagetes erecta L.) can provide worthy information for drug development. A rapid and efficient method is needed to obtain available anticancer compounds from the ethanol extract of this plant. Therefore, an offline two-dimensional preparative high performance liquid chromatography method guided by a real time cell analysis system was applied in this study. Syringic acid (1 ), quercetin (2 ), 6-hydroxykaempferol (3 ), protocatechuic acid (4 ) and quercetagetin (5 ) with high purities of over 95% were prepared using this method, while 6-hydroxykaempferol was first found in Tagetes erecta L. Compounds2, 3 and5 all were flavonoids. The compounds were evaluated for their tumor cell growth inhibitory activities on HEPG2 and A549 cells by using a real-time cell-analyzer. Four compounds were able to inhibit the proliferation of human cancer cells at a concentration of 50 μg mL −1 . Compounds2 and3 showed significant anticancer activity against A549 and HEPG2 cells. Compounds4 and5 were effective against A549 cells. The present study demonstrates that this bioassay-guided offline two-dimensional preparative high performance liquid chromatography method is very conducive to isolating plants rich in flavonoids, leading to the discovery of more worthy natural anticancer compounds.