Laser ablation and inductively coupled plasma mass spectrometry focusing on bioimaging from elemental distribution using MatLab software: a practical guide. Issue 4 (15th February 2016)

• Record Type: Journal Article
• Authors: de S. Pessôa, Gustavo; Capelo-Martínez, José L.; Fdez-Riverola, Florentino; López-Fernández, Hugo; Glez-Peña, Daniel; Reboiro-Jato, Miguel; Arruda, Marco A. Z.
• Is Part Of: Journal of analytical atomic spectrometry. Volume 31:Issue 4(2016:Apr.)
• Page Start: 832
• Page End: 840
• Publication Date: 2016-02-15
• Subjects: Atomic spectra -- Periodicals; Atomic absorption spectroscopy -- Periodicals; 543.0858
• DOI: 10.1039/c5ja00451a
• Languages: English
• ISSNs: 0267-9477

Abstract

Abstract : We discuss LA-ICP-MS in imaging elemental distribution. Abstract : The parameters influencing laser ablation inductively coupled plasma mass spectrometry as a tool for imaging elemental distribution in tissues are critically commented on in this work, and penile cancer tissue was used as a model. General aspects of LA-ICP-MS are discussed, and among them, issues regarding sample preparation and calibration. The optimization process of the following variables is described in detail in a step-by-step tutorial manner: laser intensity, laser frequency, laser resolution, ICP radiofrequency, nebulizer and auxiliary gas flow rates, and C isotopes as internal standards. Once the best condition is found for each variable, MATLAB software is used to generate two-dimensional images. Herein it is also explained how to use MATLAB software to generate tissue images using acquisition, exporting and data treatment parameters.