The effect of erythrocyte membranes from diabetic and hypercholesterolemic individuals on human carbonic anhydrase II activity. (1st January 2016)

• Record Type: Journal Article
• Authors: Yildirmis, Sermet; Alver, Ahmet; Yandi, Yunus Emre; Demir, Selim; Senturk, Ayse; Bodur, Akin; Mentese, Ahmet
• Is Part Of: Archives of physiology and biochemistry. Volume 122:Number 1(2016)
• Page Start: 14
• Page End: 18
• Publication Date: 2016-01-01
• Subjects: Erythrocyte membrane -- human carbonic anhydrase II -- hypercholesterolemia -- type 2 diabetes; Physiology -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Biochemistry; Physiology; 571
• DOI: 10.3109/13813455.2015.1111909
• Languages: English
• ISSNs: 1381-3455

Abstract

Abstract: Context : Erythrocyte membranes regulate many enzyme activities, including carbonic anhydrase II (CA II). Membrane fluidity is associated with alterations in protein function and protein-protein interactions. Objective : The purpose of this study was to show the human CA II (hCA II) activity regulation by human erythrocyte membranes from diabetic and hypercholesterolemic subjects. Materials and methods : Erythrocyte membranes were obtained from diabetic, hypercholesterolemic, and healthy subjects. hCA II activity was measured using the electrometric method. Results : hCA II activity was increased in vitro by membranes from both diabetic and hypercholesterolemic patients, with hypercholesterolemic membranes exhibiting a greater increase. Conclusion : Changes in membrane composition may affect the erythrocyte membranes' capacity to increase in vitro hCA II activity.