Converting cell fates: generating hematopoietic stem cells de novo via transcription factor reprogramming. Issue 1 (8th January 2016)

• Record Type: Journal Article
• Authors: Daniel, Michael G.; Lemischka, Ihor R.; Moore, Kateri
• Other Names: Kanz Lothar guestEditor.; Fibbe Willem E. guestEditor.; Skokowa Julia guestEditor.; Dickd John E. guestEditor.
• Is Part Of: Annals of the New York Academy of Sciences. Volume 1370:Issue 1(2016)
• Page Start: 24
• Page End: 35
• Publication Date: 2016-01-08
• Subjects: hematopoietic stem cells -- transcription factors -- reprogramming -- hematopoiesis -- cell fate conversion; Medical sciences -- Periodicals; Medicine -- Periodicals; Science -- Periodicals; 610
• DOI: 10.1111/nyas.12989
• Languages: English
• ISSNs: 0077-8923

Abstract

Abstract : Even though all paradigms of stem cell therapy and regenerative medicine emerged from the study of hematopoietic stem cells (HSCs), the inability to generate these cells de novo or expand them in vitro persists. Initial efforts to obtain these cells began with the use of embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) technologies, but these strategies have yet to yield fully functional cells. Subsequently, more recent approaches involve transcription factor (TF) overexpression to reprogram PSCs and various somatic cells. The induction of pluripotency with just four TFs by Yamanaka informs our ability to convert cell fates and demonstrates the feasibility of utilizing terminally differentiated cells to generate cells with multilineage potential. In this review, we discuss the recent efforts undertaken using TF‐based reprogramming strategies to convert several cell types into HSCs.