Γ-Glutamyl valine supplementation-induced mitigation of gut inflammation in a porcine model of colitis. (June 2016)

• Record Type: Journal Article
• Authors: Zhang, Hua; Kodera, Tomohiro; Eto, Yuzuru; Mine, Yoshinori
• Is Part Of: Journal of functional foods. Volume 24(2016)
• Page Start: 558
• Page End: 567
• Publication Date: 2016-06
• Subjects: γ-EV γ-glutamyl valine -- CaSR calcium-sensing receptor -- DSS dextran sulphate sodium -- ROS reactive oxidative species -- GI gastrointestinal -- IBD inflammatory bowel disease -- IFN interferon -- IL interleukin -- TNF tumour necrosis factor -- PBS phosphate buffer solution -- RT-PCR real-time polymer chain reaction -- PMSF phenylmethyl sulfonyl fluoride -- HBSS Hanks' balanced salt solution -- H&E haematoxylin and eosin -- β-NAD β-nicotinamide adenine dinucleotide sodium salt -- MPO myeloperoxidase; Chronic inflammation -- Colitis -- Anti-inflammatory activity -- γ-glutamyl valine; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2016.04.038
• Languages: English
• ISSNs: 1756-4646

Abstract

Highlights: Dietary dipeptide γ-glutamyl valine is an effective anti-inflammatory agent. Maintaining epithelial architecture and barrier function in porcine colitis model. Attenuating local inflammation and potentially restoring immune homeostasis. Abstract: A dietary peptide γ-glutamyl valine (EV) possesses the agonistic property to activate a calcium-sensing receptor-mediated anti-inflammatory pathway, thereby suppressing inflammation. This study aims to substantiate the therapeutic efficacy of γ-EV as an anti-inflammatory agent in a dextran sodium sulphate (DSS)-induced colitis porcine model. Colitis symptoms were observed after intra-gastric infusion of DSS for five days, followed by five days of administration of saline or γ-EV. γ-EV supplementation reduced DSS-induced weight loss and clinical signs, while preserving colonic architecture and intestinal permeability. Colonic production of tumour necrosis factor (TNF)-α and interleukin (IL)-6 were reduced to a level observed in animals without DSS treatment. The colonic gene expression of TNF-α, IL-6, IL-1β and interferon (IFN)-γ were also mitigated by γ-EV treatment, thus indicating γ-EV can restore immune homeostasis by attenuating inflammation. Our findings suggest that γ-EV supplementation may be a promising therapeutic strategy in the treatment of intestinal inflammation.