MOL1 is required for cambium homeostasis in Arabidopsis. (14th April 2016)

• Record Type: Journal Article
• Authors: Gursanscky, Nial Rau; Jouannet, Virginie; Grünwald, Karin; Sanchez, Pablo; Laaber‐Schwarz, Martina; Greb, Thomas
• Is Part Of: Plant journal. Volume 86:Number 3(2016:May)
• Page Start: 210
• Page End: 220
• Publication Date: 2016-04-14
• Subjects: stem cell niche -- cambium -- lateral plant growth -- receptor‐like kinases -- wood formation -- Arabidopsis thaliana; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.13169
• Languages: English
• ISSNs: 0960-7412

Abstract

Summary: Plants maintain pools of pluripotent stem cells which allow them to constantly produce new tissues and organs. Stem cell homeostasis in shoot and root tips depends on negative regulation by ligand–receptor pairs of the CLE peptide and leucine‐rich repeat receptor‐like kinase (LRR‐RLK) families. However, regulation of the cambium, the stem cell niche required for lateral growth of shoots and roots, is poorly characterized. Here we show that the LRR‐RLK MOL1 is necessary for cambium homeostasis in Arabidopsis thaliana . By employing promoter reporter lines, we reveal that MOL1 is active in a domain that is distinct from the domain of the positively acting CLE41/PXY signaling module. In particular, we show that MOL1 acts in an opposing manner to the CLE41/PXY module and that changing the domain or level of MOL1 expression both result in disturbed cambium organization. Underlining discrete roles of MOL1 and PXY, both LRR‐RLKs are not able to replace each other when their expression domains are interchanged. Furthermore, MOL1 but not PXY is able to rescue CLV1 deficiency in the shoot apical meristem. By identifying genes mis‐expressed in mol1 mutants, we demonstrate that MOL1 represses genes associated with stress‐related ethylene and jasmonic acid hormone signaling pathways which have known roles in coordinating lateral growth of the Arabidopsis stem. Our findings provide evidence that common regulatory mechanisms in different plant stem cell niches are adapted to specific niche anatomies and emphasize the importance of a complex spatial organization of intercellular signaling cascades for a strictly bidirectional tissue production. Significance Statement: Stem cell homeostasis in the shoot and root apical meristems is well understood, but less so in the cambium, a bifacial stem cell niche producing secondary vascular tissue. Here we show that, in analogy to other plant stem cell niches, a receptor‐like kinase, MORE LATERAL GROWTH 1 (MOL1) is a cambium‐specific repressor of stem cell activity whose expression is spatially associated with the bidirectional tissue production in the cambium. Our findings allow comparison of the regulation of anatomically distinct types of stem cell niches in plants.