Garcinone D, a natural xanthone promotes C17.2 neural stem cell proliferation: Possible involvement of STAT3/Cyclin D1 pathway and Nrf2/HO-1 pathway. (28th July 2016)

• Record Type: Journal Article
• Authors: Yang, Xiaohong; Wang, Shengnan; Ouyang, Ying; Tu, Yaling; Liu, Anmin; Tian, Yinghong; He, Mingliang; Pi, Rongbiao
• Is Part Of: Neuroscience letters. Volume 626(2016)
• Page Start: 6
• Page End: 12
• Publication Date: 2016-07-28
• Subjects: Garcinone D -- Proliferation -- Signal transducer and activator of transcription 3 -- Cyclin D1 -- Nuclear factor erythroid 2-related factor; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2016.05.012
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: Garcinone D promotes the C17.2 neural stem cell proliferation. Garcinone D increases the protein levels of phosphorylated STAT3 and Cyclin D1. Garcinone D enhances the protein levels of Nrf2 and HO-1. Nrf2 inhibitor partially reverses garcinone D-induced C17.2 cell proliferation. Abstract: Garcinia mangostana L. (Mangosteen) has been used to treat various pathological conditions, including inflammation and urinary tract infections. Here, we observed that garcinone D, a natural xanthone from mangosteen, promoted the proliferation of C17.2 neural progenitor cells and also resulted in a larger percentage of cells in S phase compared with the control group. Moreover, garcinone D increased the protein levels of phosphorylated signal transducer and activator of transcription 3 (p-STAT3) and Cyclin D1 in concentration- and time- dependent manners. Garcinone D also increased the protein levels of nuclear factor erythroid 2-related factor (Nrf2) and heme oxygenase-1 (HO-1) in concentration- and time- dependent manners, and inhibiting Nrf2 activation by brusatol could partly reverse garcinone D-induced C17.2 cell proliferation. Taken together, it is the first time to show that garcinone D promotes the proliferation of C17.2 neural stem cells, which may involve the STAT3/Cyclin D1 pathway and Nrf2/HO-1 pathway. It would provide new inspiration to develop garcinone D as a lead compound to promote the proliferation of endogenous neural stem cells (NSCs).