CCL11‐induced eosinophils inhibit the formation of blood vessels and cause tumor necrosis. (12th May 2016)

• Record Type: Journal Article
• Authors: Xing, Yanjiang; Tian, Yijun; Kurosawa, Takamasa; Matsui, Sayaka; Touma, Maki; Yanai, Takanori; Wu, Qiong; Sugimoto, Kenkichi
• Is Part Of: Genes to cells. Volume 21:Number 6(2016)
• Page Start: 624
• Page End: 638
• Publication Date: 2016-05-12
• Subjects: Cytogenetics -- Periodicals; Cells -- Mechanical properties -- Periodicals; Molecular genetics -- Periodicals; Genes -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Biomechanics -- Periodicals; 571.6
• DOI: 10.1111/gtc.12371
• Languages: English
• ISSNs: 1356-9597

Abstract

Abstract : We previously demonstrated that IL‐18 and CCL11 were highly expressed in an NFSA tumor cell line that showed limited angiogenesis and severe necrosis. However, IL‐18 was not responsible for the immune cell accumulation and necrosis. Here, we attempted to clarify the relevance of CCL11 in angiogenesis and tumor formation. We established CCL11‐overexpressing MS‐K cell clones (MS‐K‐CCL11) to assess the role of CCL11 in immune cell accumulation and angiogenesis. The MS‐K‐CCL11 cells did not form tumors in mice. MS‐K‐CCL11‐conditioned medium (CM) and recombinant CCL11 induced macrophage and eosinophil differentiation from bone marrow cells. The MS‐K‐CCL11‐CM effectively recruited the differentiated eosinophils. Furthermore, the eosinophils damaged the MS‐K, NFSA and endothelial cells in a dose‐dependent manner. Administration of an antagonist of CCR3, a CCL11 receptor, to NFSA tumor‐bearing mice restored the blood vessel formation and blocked the eosinophil infiltration into the NFSA tumors. Furthermore, other CCL11‐overexpressing LM8 clones were established, and their tumor formation ability was reduced compared to the parental LM8 cells, accompanied by increased eosinophil infiltration, blockade of angiogenesis and necrosis. These results indicate that CCL11 was responsible for the limited angiogenesis and necrosis by inducing and attracting eosinophils in the tumors. Abstract : MS‐K‐CCL11‐conditioned medium (CM) and recombinant CCL11 induced eosinophil differentiation and effectively recruited the differentiated eosinophils. The eosinophils damaged the MS‐K, NFSA and endothelial cells in a dose‐dependent manner. CCL11 was responsible for the limited angiogenesis and necrosis by inducing and attracting eosinophils in the tumors.