Gonadotropin‐releasing hormone analogues lead to pro‐inflammatory changes in T lymphocytes. (July 2016)

• Record Type: Journal Article
• Authors: Sung, Nayoung; Salazar García, María D.; Dambaeva, Svetlana; Beaman, Kenneth D.; Gilman‐Sachs, Alice; Kwak‐Kim, Joanne
• Is Part Of: American journal of reproductive immunology. Volume 76:Number 1(2016)
• Page Start: 50
• Page End: 58
• Publication Date: 2016-07
• Subjects: gonadotropin‐releasing hormone (GnRH) -- GnRH agonist -- GnRH antagonist -- T helper (TH) 1 immunity -- TH17; Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.12522
• Languages: English
• ISSNs: 1046-7408

Abstract

Abstract : Problem: We aimed to investigate the effect of gonadotropin‐releasing hormone (GnRH) analogues on T‐cell immunity. Method of study: TNF‐α + ‐, INF‐ɣ + ‐, IL‐10 + ‐, and IL‐17 + ‐expressing T cells in peripheral blood mononuclear cells (PBMCs) were treated with various concentrations (0.1, 1, 5, and 10 μm ) of GnRH agonist (buserelin acetate) and antagonist (cetrorelix acetate) for 4 hours in vitro and they were analyzed with flow cytometry. Results: TNF‐α + /IL‐10 + T helper (TH) cell ratios were increased in PBMCs treated with 1, 5, and 10 μm GnRH agonist when compared to controls ( P =.006, P =.014 and P =.030, respectively). IFN‐ɣ + /IL‐10 + TH cell ratios were significantly increased with 0.1, 1, 5, and 10 μm GnRH agonist as compared with controls ( P =.046, P =.004, P =.013, and P =.011, respectively). TNF‐α + TH cell levels, and IFN‐γ + /IL‐10 + TH cell ratios were significantly different ( P <.001 and P <.004, respectively) between GnRH agonist‐ and antagonist‐treated cells. Conclusion: GnRH analogues induce pro‐inflammatory TH1 shift in T‐cell immunity, in vitro. GnRH treatment during assisted reproductive technology cycle might explain a possible cause of inflammatory flare in women with inflammatory conditions.