Identification and functional characterization of a glucose regulated protein 94 gene in Litopenaeus vannamei and its responsiveness in WSSV infection. (May 2016)

• Record Type: Journal Article
• Authors: Bi, Hai-Tao; Yuan, Feng-Hua; Yuan, Kai; Weng, Shao-Ping; He, Jian-Guo; Chen, Yi-Hong
• Is Part Of: Molecular immunology. Volume 73(2016:May)
• Page Start: 29
• Page End: 36
• Publication Date: 2016-05
• Subjects: Litopenaeus vannamei -- ER-stress -- Unfolded protein response -- White spot syndrome virus -- Glucose regulated protein 94; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2016.03.012
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: Identification and characterization of a glucose regulated protein 94 in Litopenaeus vannamei (designated as LvGRP94 ). Promoter of LvGRP94 was activated by L. vannamei X-box binding protein 1 or heat shock treatment in S2 cells. Expression of LvGRP94 was induced by WSSV infection or unfolded protein response pathway activation. Knocked-down LvGRP94 reduced the mortality of L. vannamei upon WSSV infection. Abstract: In the current study, a cDNA of glucose regulated protein 94 ( LvGRP94 ) was cloned from Litopenaeus vannamei . Subcellular localization assay revealed that LvGRP94 expressed in endoplasmic reticulum (ER). And results of reported gene assays demonstrated that the promoter of LvGRP94 was activated by L . vannamei leucine zipper domain transcription factor X-box binding protein 1 (LvXBP1) or heat shock treatment. Furthermore, LvGRP94 was found to highly express in hemocytes as well as in epidermis by real-time RT-PCR. In addition, it was shown that LvGRP94 inhibited by LvXBP1 knocked-down in the hemocytes, was induced by white spot syndrome virus (WSSV) infection, or unfolded protein response (UPR) pathway activation. Importantly, decreasing LvGRP94 reduced the cumulative mortality of WSSV-infected shrimps and WSSV copies in shrimp muscle. These results suggested that LvGRP94 might involve in shrimp UPR pathway as well as WSSV infection.