Internalization and cytotoxicity effects of carbon-encapsulated iron nanoparticles in murine endothelial cells: Studies on internal dosages due to loaded mass agglomerates. (August 2016)

• Record Type: Journal Article
• Authors: Cywinska, Monika A.; Bystrzejewski, Michal; Poplawska, Magdalena; Kosmider, Anita; Zdanowski, Robert; Lewicki, Slawomir; Fijalek, Zbigniew; Ostrowska, Agnieszka; Bamburowicz, Magdalena; Cieszanowski, Andrzej; Grudzinski, Ireneusz P.
• Is Part Of: Toxicology in vitro. Volume 34(2016)
• Page Start: 229
• Page End: 236
• Publication Date: 2016-08
• Subjects: Iron-carbon (core-shell) type hybrid nanoparticles -- Endothelial (HECa-10) cells -- Agglomerates -- Internalization -- Zeta potential -- Cytotoxicity; Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2016.04.011
• Languages: English
• ISSNs: 0887-2333

Abstract

Abstract: Carbon-encapsulated iron nanoparticles (CEINs) qualified as metal-inorganic hybrid nanomaterials offer a potential scope for an increasing number of biomedical applications. In this study, we have focused on the investigation of cellular fate and resulting cytotoxic effects of CEINs synthesized using a carbon arc route and studied in murine endothelial (HECa-10) cells. The CEIN samples were characterized as pristine (the mean diameter between 47 and 56 nm) and hydrodynamic (the mean diameter between 270 and 460 nm) forms and tested using a battery of methods to determine the cell internalization extent and cytotoxicity effects upon to the exposures (0.0001–100 μg/ml) in HECa-10 cells. Our studies evidenced that the incubation with CEINs for 24 h is accompanied with substantial changes of Zeta potential in cells which can be considered as a key factor for affecting the membrane transport, cellular distribution and cytotoxicity of these nanoparticles. The results demonstrate that CEINs have entered the endothelial cell through the endocytic pathway rather than by passive diffusion and they were mainly loaded as agglomerates on the cell membrane and throughout the cytoplasm, mitochondria and nucleus. The studies show that CEINs induce the mitochondrial and cell membrane cytotoxicities in a dose-dependent manner resulting from the internal dosages due to CEIN agglomerates. Our results highlight the importance of the physicochemical characterization of CEINs in studying the magnetic nanoparticle–endothelial cell interactions because the CEIN mass agglomerates can sediment more or less rapidly in culture models. Graphical abstract: The main targets of carbon-encapsulated iron nanoparticles (CEINs)-induced cytotoxicity in murine endothelial (HECa-10) cells. The black circles are CEINs. Highlights: Carbon-encapsulated iron nanoparticles (CEINs) loaded as agglomerates in HECa-10. CEIN agglomerates enter the endothelial cells through the endocytic pathway. CEIN agglomerates affect the Zeta potential of HECa-10 cells. CEIN agglomerates induce membrane and mitochondrial cytotoxicities in HECa-10 cells.