PH and temperature stability of (−)-epigallocatechin-3-gallate-β-cyclodextrin inclusion complex-loaded chitosan nanoparticles. (20th September 2016)

• Record Type: Journal Article
• Authors: Liu, Fei; Majeed, Hamid; Antoniou, John; Li, Yue; Ma, Yun; Yokoyama, Wallace; Ma, Jianguo; Zhong, Fang
• Is Part Of: Carbohydrate polymers. Volume 149(2016)
• Page Start: 340
• Page End: 347
• Publication Date: 2016-09-20
• Subjects: (−)-Epigallocatechin-3-gallate (PubChem CID: 65064) -- Chitosan hydrochloride (PubChem CID: 71853) -- Sulfobutylether-β-cyclodextrin sodium (PubChem CID: 66577045); EGCG (−)-Epigallocatechin-3-gallate -- CSH chitosan hydrochloride -- SBE-β-CD sulfobutylether-β-cyclodextrin sodium -- CD cyclodextrin -- CSN CSH-SBE-β-CD nanoparticle -- DPPH 2, 2-diphenyl-1-picrylhydrazyl; (−)-Epigallocatechin-3-gallate -- Sulfobutylether-β-cyclodextrin -- Inclusion complexes -- Chitosan nanoparticles -- Stability -- Antioxidant activity; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2016.04.100
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Possible changes of EGCG-loaded CSNs and EGCG-ICs after altering the pH value or temperature of nanoparticle suspensions. Highlights: Oxidative stability of EGCG incorporated into ICs and CSNs were both investigated. EGCG-ICs and related CSNs were physically unstable at higher pH and temperature. Antioxidant activities of CSNs decreased more compared to EGCG-ICs and free EGCG. Extreme entrapment of EGCG in aggregated CSNs inhibited the antioxidant activities. Nanostructure of CSNs was lost but the activity of encapsulated EGCG may remain. Abstract: The oxidative stability of (−)-epigallocatechin-3-gallate (EGCG) incorporated as inclusion complexes (ICs) in sulfobutylether-β-cyclodextrin sodium (SBE-β-CD) and then ionotropically crosslinked with chitosan hydrochloride (CSH) into nanoparticles were investigated. EGCG-loaded CSH-SBE-β-CD nanoparticles (CSNs) were physically unstable at higher pH and temperature. The particle size of CSNs was unchanged in the pH range of 3–5, but the microenvironment of EGCG-IC appeared to be intact until the pH increased to 6.5 by fluorescence spectroscopy. The physical structure of EGCG-ICs was also affected during storage in addition to CSNs, which was further affected as temperature increased from 25 to 55 °C. The decrease in antioxidant activities of EGCG-ICs and free EGCG with increasing pH, storage time and temperature were modest compared to the prominent decreases in antioxidant activities of EGCG-loaded CSNs. The extreme entrapment of EGCG-ICs and/or free EGCG in the aggregated CSNs restricted the release of EGCG, thus inhibiting the antioxidant activities.