Clusters of Phospholipid Vesicles as Platforms for Glucose Oxidase‐Catalyzed Reaction in a Bubble‐Column Bioreactor. Issue 6 (7th April 2016) - Yoshimoto, Makoto
Abstract: Clusters of negatively charged liposomes encapsulated with glucose oxidase were prepared in the presence of Ca 2+ and used to catalyze the oxidation of glucose in an external loop airlift bubble column. The clusters exhibited higher catalytic activity compared to nonclustered glucose oxidase‐containing liposomes (GOLs) when the liposome membranes were incorporated with cholesterol. The clusters were structurally altered in shear flow to give sufficient interfacial area accessible to glucose. The reactivity of GOL clusters could be modulated on the basis of lipid composition of the membranes which affected the mode of interaction among liposomes through Ca 2+ . Part of GOL clusters could be separated from the reaction mixture by centrifugation, which would be advantageous for reusing liposomal catalysts. The liposome clusters can be the platforms to regulate the catalytic performance of glucose oxidase in the airlift. Abstract : The clusters of liposomes encapsulated with glucose oxidase serve as catalysts for oxidation of glucose in an airlift bubble column. Structural flexibility of the liposome clusters in shear flow allows efficient transfer of glucose from the bulk liquid to the interior of liposomes. The enzyme‐containing liposome clusters are useful as reactive and recoverable catalysts in practical bioreactors.