Pharmacokinetic study of dendrobine in rat plasma by ultra‐performance liquid chromatography tandem mass spectrometry. (25th November 2015)

• Record Type: Journal Article
• Authors: Wang, Shuanghu; Wu, Haiya; Geng, Peiwu; Lin, Yingying; Liu, Zezheng; Zhang, Lijing; Ma, Jianshe; Zhou, Yunfang; Wang, Xianqin; Wen, Congcong
• Is Part Of: Biomedical chromatography. Volume 30:Number 7(2016:Jul.)
• Page Start: 1145
• Page End: 1149
• Publication Date: 2015-11-25
• Subjects: dendrobine -- UPLC‐MS/MS -- pharmacokinetics -- rat plasma; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3641
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: Dendrobine, considered as the major active alkaloid compound, has been used for the quality control and discrimination of Dendrobium which is documented in the Chinese Pharmacopoeia . In this work, a sensitive and simple ultra‐performance liquid chromatography tandem mass spectrometry (UPLC‐MS/MS) method for determination of dendrobine in rat plasma is developed. After addition of caulophyline as an internal standard (IS), protein precipitation by acetonitrile–methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C18 (2.1 ×100 mm, 1.7 µm) column with acetonitrile and 0.1% formic acid as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions m / z 264.2 → 70.0 for dendrobine and m / z 205.1 → 58.0 for IS. Calibration plots were linear throughout the range 2–1000 ng/mL for dendrobine in rat plasma. The RSDs of intra‐day and inter‐day precision were both <13%. The accuracy of the method was between 95.4 and 103.9%. The method was successfully applied to pharmacokinetic study of dendrobine after intravenous administration. Copyright © 2015 John Wiley & Sons, Ltd.