Developmental validation of STRmix™, expert software for the interpretation of forensic DNA profiles. (July 2016)

• Record Type: Journal Article
• Authors: Bright, Jo-Anne; Taylor, Duncan; McGovern, Catherine; Cooper, Stuart; Russell, Laura; Abarno, Damien; Buckleton, John
• Is Part Of: Forensic science international. Volume 23(2016:Jul.)
• Page Start: 226
• Page End: 239
• Publication Date: 2016-07
• Subjects: DNA mixtures -- Probabilistic genotyping -- Continuous method -- Validation -- STRmix; Forensic genetics -- Periodicals; Génétique légale -- Périodiques; Forensic genetics; Electronic journals; Periodicals; 614.1
• DOI: 10.1016/j.fsigen.2016.05.007
• Languages: English
• ISSNs: 1872-4973

Abstract

Highlights: The developmental validation of STRmix™ following the 2015 SWGDAM Guidelines for the Validation of Probabilistic Genotyping Systems is described. Performance of the software is discussed with respect to sensitivity, specificity, precision, accuracy, and reproducibility. Abstract: In 2015 the Scientific Working Group on DNA Analysis Methods published the SWGDAM Guidelines for the Validation of Probabilistic Genotyping Systems [1]. STRmix™ is probabilistic genotyping software that employs a continuous model of DNA profile interpretation. This paper describes the developmental validation activities of STRmix™ following the SWGDAM guidelines. It addresses the underlying scientific principles, and the performance of the models with respect to sensitivity, specificity and precision and results of interpretation of casework type samples. This work demonstrates that STRmix™ is suitable for its intended use for the interpretation of single source and mixed DNA profiles.