Towards Process Spectroscopy in Complex Fermentation Samples and Mixtures. Issue 6 (7th April 2016)

• Record Type: Journal Article
• Authors: Paul, Andrea; Carl, Peter; Westad, Frank; Voss, Jan‐Patrick; Maiwald, Michael
• Other Names: Maiwald Michael sponsoringEditor.
• Is Part Of: Chemie Ingenieur Technik. Volume 88:Issue 6(2016:Jun.)
• Page Start: 756
• Page End: 763
• Publication Date: 2016-04-07
• Subjects: Fluorescence -- NIR spectroscopy -- Partial least squares regression -- Pichia pastoris -- Raman spectroscopy -- UV/VIS spectroscopy; Chemical engineering -- Patents -- Periodicals; Chemical engineering -- Periodicals; Chemical industry -- Periodicals; Chemistry, Technical -- Periodicals; 660.05
• DOI: 10.1002/cite.201500118
• Languages: English
• ISSNs: 0009-286X

Abstract

Abstract: Supernatants from a fermentation process of Pichia pastoris were investigated by Raman spectroscopy. Using partial least squares regression, the principal substrates glycerol and methanol could be predicted, however not the expressed protein. To gain further insight, a priori prepared calibration samples were studied by vibrational‐, UV/Vis‐, and fluorescence spectroscopy. For the quantification of glycerol and methanol, Raman spectroscopy was identified as the most sensitive technique, and superior to near‐infrared spectroscopy, but not for protein contents below 1 g L −1 . Both UV/Vis absorption and fluorescence spectroscopy are well suited for the quantification of protein, however, best results were obtained with UV/Vis absorption. Abstract : Contents of glycerol, methanol and the expressed protein in supernatants from a fermentation process of Pichia pastoris were investigated by Raman spectroscopy and quantified by partial least squares regression. Furthermore, the potential of Raman, NIR spectroscopy, UV/VIS and 2D fluorescence spectroscopic sensors for a priory samples is discussed.