AsHSP17, a creeping bentgrass small heat shock protein modulates plant photosynthesis and ABA‐dependent and independent signalling to attenuate plant response to abiotic stress. (12th February 2016)

• Record Type: Journal Article
• Authors: Sun, Xinbo; Sun, Chunyu; Li, Zhigang; Hu, Qian; Han, Liebao; Luo, Hong
• Is Part Of: Plant, cell and environment. Volume 39:Number 6(2016)
• Page Start: 1320
• Page End: 1337
• Publication Date: 2016-02-12
• Subjects: creeping bentgrass -- ABA -- abiotic stress -- AsHSP17 -- RNA‐seq -- salt tolerance -- small heat shock protein; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.12683
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: Heat shock proteins (HSPs) are molecular chaperones that accumulate in response to heat and other abiotic stressors. Small HSPs (sHSPs) belong to the most ubiquitous HSP subgroup with molecular weights ranging from 12 to 42 kDa. We have cloned a new sHSP gene, AsHSP17 from creeping bentgrass ( Agrostis stolonifera ) and studied its role in plant response to environmental stress. AsHSP17 encodes a protein of 17 kDa. Its expression was strongly induced by heat in both leaf and root tissues, and by salt and abscisic acid (ABA) in roots. Transgenic Arabidopsis plants constitutively expressing AsHSP17 exhibited enhanced sensitivity to heat and salt stress accompanied by reduced leaf chlorophyll content and decreased photosynthesis under both normal and stressed conditions compared to wild type. Overexpression of AsHSP17 also led to hypersensitivity to exogenous ABA and salinity during germination and post‐germinative growth. Gene expression analysis indicated that AsHSP17 modulates expression of photosynthesis‐related genes and regulates ABA biosynthesis, metabolism and ABA signalling as well as ABA‐independent stress signalling. Our results suggest that AsHSP17 may function as a protein chaperone to negatively regulate plant responses to adverse environmental stresses through modulating photosynthesis and ABA‐dependent and independent signalling pathways. Abstract : Heat shock proteins (HSPs) are molecular chaperones that accumulate in response to environmental stress. A new small HSP (sHSP) gene, AsHSP17 from creeping bentgrass ( Agrostis stolonifera ) has been cloned and shown to negatively regulate plant response to heat and salt stress through modulating photosynthesis and ABA‐dependent and independent signaling pathways. Data obtained in this study provide new insight into the plant stress regulatory pathways and a better understanding of the role that sHSPs play in that response.