Preconcentration of valsartan by dispersive liquid–liquid microextraction based on solidification of floating organic drop and its determination in urine sample: Central composite design. Issue 10 (2nd May 2016)

• Record Type: Journal Article
• Authors: Pebdani, Arezou Amiri; Shabani, Ali Mohammad Haji; Dadfarnia, Shayesteh; Talebianpoor, Mohammad Sharif; Khodadoust, Saeid
• Is Part Of: Journal of separation science. Volume 39:Issue 10(2016)
• Page Start: 1935
• Page End: 1944
• Publication Date: 2016-05-02
• Subjects: Central composite design -- Microextraction -- Solidification -- Urine -- Valsartan; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201600010
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : In this work, a fast, easy, and efficient dispersive liquid–liquid microextraction method based on solidification of floating organic drop followed by high‐performance liquid chromatography with UV detection was developed for the separation/preconcentration and determination of the drug valsartan. Experimental design was applied for the optimization of the effective variables (such as volume of extracting and dispersing solvents, ionic strength, and pH) on the extraction efficiency of valsartan from urine samples. The optimized values were 250.0 μL ethanol, 65.0 μL 1‐dodecanol, 4.0% w/v NaCl, pH 3.8, 1.0 min extraction time, and 4.0 min centrifugation at 4000 rpm min −1 . The linear response ( r 2 = 0.997) was obtained in the range of 0.013–10.0 μg mL −1 with a limit of detection of 4.0 ng mL −1 and relative standard deviations of less than 5.0 % ( n = 6).