Two single nucleotide polymorphisms in the promoter of the ovine myostatin gene (MSTN) and their effect on growth and carcass muscle traits in New Zealand Romney sheep. (16th June 2015)

• Record Type: Journal Article
• Authors: Wang, J.; Zhou, H.; Hu, J.; Li, S.; Luo, Y.; Hickford, J.G.H.
• Is Part Of: Journal of animal breeding and genetics. Volume 133:Number 3(2016)
• Page Start: 219
• Page End: 226
• Publication Date: 2015-06-16
• Subjects: Carcass muscle traits -- growth traits -- MSTN -- NZ Romney lambs -- PCR–SSCP -- promoter SNP; Livestock -- Breeding -- Periodicals; Livestock -- Genetics -- Periodicals; 636.0820
• DOI: 10.1111/jbg.12171
• Languages: English
• ISSNs: 0931-2668

Abstract

Summary: Myostatin is a negative regulator of muscle growth and development in mammals, and variation in ovine myostatin gene ( MSTN ) has been demonstrated to be associated with variation in the muscularity of sheep. Polymerase chain reaction–single‐stranded conformational polymorphism (PCR–SSCP) was used to look for single nucleotide polymorphisms (SNPs) in a 304‐bp amplicon from the promoter region of ovine MSTN . Sequence analyses revealed two previously identified SNPs (c.−2449G/C and c. −2379T/C) that resulted in three haplotypes ( H 1 (c.[−2449G; −2379C]), H 2 (c.[−2449C; −2379C]) and H 3 (c.[−2449G; −2379T]). The effect of these SNPs on growth and carcass traits was investigated in 357 NZ Romney lambs. General linear mixed‐effect models revealed that sheep with the genotype c.−2449GC had a higher loin meat yield (p = 0.032) and proportion loin yield (p = 0.028), than those with the genotype c.−2449GG. The genotype c.−2379CC was associated with an increase in three weight traits: birthweight (p = 0.003), tailing weight (p = 0.009) and weaning weight (p = 0.028), when compared with the genotype c.−2379TC, but it was not found to have an association with growth rate. This suggests that c.−2379T/C has an effect that originates at, or before birth. Haplotype H 3 was associated with a decrease in birthweight (p = 0.002), tailing weight (p = 0.003) and weaning weight (p = 0.011). Haplotype H 2 was associated with increased loin yield (p = 0.012) and proportion loin yield (p = 0.002). The SNPs may have value as genetic markers for improved Romney breeding.