Cell Phenotyping: Multiparametric Biomechanical and Biochemical Phenotypic Profiling of Single Cancer Cells Using an Elasticity Microcytometer (Small 17/2016). Issue 17 (May 2016)

• Record Type: Journal Article
• Authors: Hu, Shuhuan; Liu, Guangyu; Chen, Weiqiang; Li, Xiang; Lu, Wei; Lam, Raymond H. W.; Fu, Jianping
• Is Part Of: Small. Volume 12:Issue 17(2016)
• Page Start: 2247
• Page End: 2247
• Publication Date: 2016-05
• Subjects: cell phenotyping -- cell deformability -- surface proteins -- microfluidics -- elasticity microcytometers; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201670084
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract : An elasticity microcytometer capable of multiparametric biomechanical and biochemical phenotypic profiling of single cancer cells is developed to interpret cell deformation along antibody‐coated confining microchannels as quantitative cell characteristics including cell size, cell deformability/stiffness, and expression levels of surface receptors simultaneously for the same single live cancer cells. This elasticity microcytometer, developed by R. H. W. Lam, J. Fu and co‐workers on page 2300, holds a great promise for comprehensive molecular, cellular, and biomechanical phenotypic profiling of live cancer cells at the single cell level.