Radial glia inhibit peripheral glial infiltration into the spinal cord at motor exit point transition zones. Issue 7 (31st March 2016)

• Record Type: Journal Article
• Authors: Smith, Cody J.; Johnson, Kimberly; Welsh, Taylor G.; Barresi, Michael J. F.; Kucenas, Sarah
• Is Part Of: Glia. Volume 64:Issue 7(2016:Jul.)
• Page Start: 1138
• Page End: 1153
• Publication Date: 2016-03-31
• Subjects: motor exit point glia -- CNS/PNS transition zone -- radial glia -- zebrafish -- neural crest cells; Neuroglia -- Periodicals; Neurology -- Periodicals; 611.0188
• DOI: 10.1002/glia.22987
• Languages: English
• ISSNs: 0894-1491

Abstract

Abstract : In the mature vertebrate nervous system, central and peripheral nervous system (CNS and PNS, respectively) GLIA myelinate distinct motor axon domains at the motor exit point transition zone (MEP TZ). How these cells preferentially associate with and myelinate discrete, non‐overlapping CNS versus PNS axonal segments, is unknown. Using in vivo imaging and genetic cell ablation in zebrafish, we demonstrate that radial glia restrict migration of PNS glia into the spinal cord during development. Prior to development of radial glial endfeet, peripheral cells freely migrate back and forth across the MEP TZ. However, upon maturation, peripherally located cells never enter the CNS. When we ablate radial glia, peripheral glia ectopically migrate into the spinal cord during developmental stages when they would normally be restricted. These findings demonstrate that radial glia contribute to both CNS and PNS development and control the unidirectional movement of glial cell types across the MEP TZ early in development. GLIA 2016. GLIA 2016;64:1138–1153 Main Points: Radial glia function to restrict ectopic migration of peripheral cells into the spinal cord. Interestingly, upon their ablation, central cell populations are never observed ectopically exiting the CNS.