Time lapse in vivo microscopy reveals distinct dynamics of microglia‐tumor environment interactions—a new role for the tumor perivascular space as highway for trafficking microglia. Issue 7 (3rd May 2016)

• Record Type: Journal Article
• Authors: Bayerl, Simon Heinrich; Niesner, Raluca; Cseresnyes, Zoltan; Radbruch, Helena; Pohlan, Julian; Brandenburg, Susan; Czabanka, Marcus Alexander; Vajkoczy, Peter
• Is Part Of: Glia. Volume 64:Issue 7(2016:Jul.)
• Page Start: 1210
• Page End: 1226
• Publication Date: 2016-05-03
• Subjects: glioma -- microglia motility -- 2 photon microscopy -- NADPH oxidase -- angiogenesis; Neuroglia -- Periodicals; Neurology -- Periodicals; 611.0188
• DOI: 10.1002/glia.22994
• Languages: English
• ISSNs: 0894-1491

Abstract

Abstract : Microglial cells are critical for glioma growth and progression. However, only little is known about intratumoral microglial behavior and the dynamic interaction with the tumor. Currently the scarce understanding of microglial appearance in malignant gliomas merely originates from histological studies and in vitro investigations. In order to understand the pattern of microglia activity, motility and migration we designed an intravital study in an orthotopic murine glioma model using CX3CR1‐eGFP GFP/wt mice. We analysed the dynamics of intratumoral microglia accumulation and activity, as well as microglia/tumor blood vessel interaction by epi‐illumination and 2‐photon laser scanning microscopy. We further investigated cellular and tissue function, including the enzyme activity of intratumoral and microglial NADPH oxidase measured by in vivo fluorescence lifetime imaging. We identified three morphological phenotypes of tumor‐associated microglia cells with entirely different motility patterns. We found that NADPH oxidase activation is highly divergent in these microglia subtypes leading to different production levels of reactive oxygen species (ROS). We observed that microglia motility is highest within the perivascular niche, suggesting relevance of microglia/tumor blood vessel interactions. In line, reduction of tumor blood vessels by antivascular therapy confirmed the relevance of the tumor vessel compartment on microglia biology in brain tumors. In summary, we provide new insights into in vivo microglial behavior, regarding both morphology and function, in malignant gliomas. GLIA 2016;64:1210–1226 Main Points: We newly discovered three different phenotypes of glioma‐associated microglia with individual functions. We gave a new insight in the crucial role of the microglia‐vascular interplay and of the vascular tumor network for microglia migration.