Effects of Narrow‐band IR‐A and of Water‐Filtered Infrared A on Fibroblasts. (17th March 2016)

• Record Type: Journal Article
• Authors: Knels, Lilla; Valtink, Monika; Piazena, Helmut; de la Vega Marin, Jamlec; Gommel, Kerstin; Lupp, Amelie; Roehlecke, Cora; Mehner, Mirko; Funk, Richard H. W.
• Is Part Of: Photochemistry and photobiology. Volume 92:Number 3(2016)
• Page Start: 475
• Page End: 487
• Publication Date: 2016-03-17
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.12579
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: Exposures of the skin with electromagnetic radiation of wavelengths between 670 nm and 1400 nm are often used as a general treatment to improve wound healing and reduce pain, for example, in chronic diabetic skin lesions. We investigated the effects of water‐filtered infrared A (wIRA) and of narrow‐band IR‐A provided by a light‐emitting diode LED (LED‐IR‐A) irradiation in vitro on 3T3 fibroblast cultures under defined conditions with and without glyoxal administration. Glyoxal triggers the formation of advanced glycation end products, thereby mimicking a diabetic metabolic state. Cell viability and apoptotic changes were determined by flow cytometry after vital staining with Annexin V, YO‐PRO‐1 and propidium iodide (PI), and by SubG1 assay. Mitochondrial function and oxidative stress were examined by vital staining for radical production, mitochondrial membrane potential (MMP) and the ratio of reduced‐to‐oxidized glutathione (GSH/GSSG). The metabolic state was monitored by a resazurin conversion assay. The numbers of apoptotic cells were reduced in cultures irradiated with wIRA or LED‐IR‐A. More mitochondria showed a well‐polarized MMP after wIRA irradiation in glyoxal damaged cells. LED‐IR‐A treatment specifically restored the GSH/GSSG ratio. The immediate positive effects of wIRA and LED‐IR‐A observed in living cells, particularly on mitochondria, reflect the therapeutic benefits of wIRA and LED‐IR‐A. Abstract : Irradiating the skin with electromagnetic radiation of wavelengths between 670 nm and 1400 nm is often used to improve wound healing and reduce pain. We investigated the effects of water‐filtered infrared‐A (wIRA) and of light‐emitting diode (LED)‐emitted narrow‐band IR‐A (LED‐IR‐A) on 3T3 fibroblast cultures. Glyoxal was added to mimic a diabetic metabolic state. We observed that IR‐A treatment enhanced the vital status of the cells, especially the oxidative status. The immediate positive effects of wIRA and LED‐IR‐A observed in living cells, particularly on their mitochondria, reflect the therapeutic benefits of wIRA and LED‐IR‐A.