Nanocarbon Allotropes—Graphene and Nanocrystalline Diamond—Promote Cell Proliferation. Issue 18 (22nd March 2016)

• Record Type: Journal Article
• Authors: Verdanova, Martina; Rezek, Bohuslav; Broz, Antonin; Ukraintsev, Egor; Babchenko, Oleg; Artemenko, Anna; Izak, Tibor; Kromka, Alexander; Kalbac, Martin; Hubalek Kalbacova, Marie
• Is Part Of: Small. Volume 12:Issue 18(2016)
• Page Start: 2499
• Page End: 2509
• Publication Date: 2016-03-22
• Subjects: carbon allotropes -- cell adhesion -- cell proliferation -- graphene -- nanocrystalline diamonds; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201503749
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract : Two profoundly different carbon allotropes – nanocrystalline diamond and graphene – are of considerable interest from the viewpoint of a wide range of biomedical applications including implant coating, drug and gene delivery, cancer therapy, and biosensing. Osteoblast adhesion and proliferation on nanocrystalline diamond and graphene are compared under various conditions such as differences in wettability, topography, and the presence or absence of protein interlayers between cells and the substrate. The materials are characterized in detail by means of scanning electron microscopy, atomic force microscopy, photoelectron spectroscopy, Raman spectroscopy, and contact angle measurements. In vitro experiments have revealed a significantly higher degree of cell proliferation on graphene than on nanocrystalline diamond and a tissue culture polystyrene control material. Proliferation is promoted, in particular, by hydrophobic graphene with a large number of nanoscale wrinkles independent of the presence of a protein interlayer, i.e., substrate fouling is not a problematic issue in this respect. Nanowrinkled hydrophobic graphene, thus, exhibits superior characteristics for those biomedical applications where high cell proliferation is required under differing conditions. Abstract : Nanowrinkled hydrophobic graphene extensively promotes osteoblast proliferation regardless of the presence of fetal bovine serum. On the contrary, nanorough hydrophobic and hydrophilic nanocrystalline diamond films and flat hydrophilic graphene perform in that respect similarly to commonly used tissue culture plastic while providing other useful functions. These nanoscale carbon allotropes can thus provide features interesting for various biomedical research and applications.