Mean squared displacement from fluorescence correlation spectroscopy. Issue 16 (21st March 2016)

• Record Type: Journal Article
• Authors: Kubečka, Jakub; Uhlík, Filip; Košovan, Peter
• Is Part Of: Soft matter. Volume 12:Issue 16(2016)
• Page Start: 3760
• Page End: 3769
• Publication Date: 2016-03-21
• Subjects: Soft condensed matter -- Periodicals; 530.413
• DOI: 10.1039/c6sm00296j
• Languages: English
• ISSNs: 1744-683X

Abstract

Abstract : MSD from FCS? Abstract : Under certain conditions, the mean squared displacement (MSD) can be retrieved from fluorescence correlation spectroscopy (FCS) measurements. However, in the general case this procedure is not valid, and the apparent MSD obtained from FCS data may substantially differ from the true one. In this work we discuss under which conditions this procedure can be applied. Furthermore, we use computer simulations to obtain the MSD and the apparent MSD for the diffusion of a single polymer chain under various approximations. Based on the simulation results we discuss the reliability of the apparent MSD obtained from FCS, showing that it systematically deviates from the true MSD. We also propose a general procedure to verify the reliability of the apparent MSD by measurements at various focal spot sizes.