Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans. Issue 17 (15th April 2016)

• Record Type: Journal Article
• Authors: Calderon, Angie D.; Liu, Yunpeng; Li, Xu; Wang, Xuan; Chen, Xi; Li, Lei; Wang, Peng G.
• Is Part Of: Organic & biomolecular chemistry. Volume 14:Issue 17(2016)
• Page Start: 4027
• Page End: 4031
• Publication Date: 2016-04-15
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c6ob00586a
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : Based on substrate specificity of human FUT8, an efficient strategy was developed for the synthesis of core-fucosylated asymmetric N -glycans. Abstract : Substrate specificity studies of human FUT8 using 77 structurally-defined N -glycans as acceptors showed a strict requirement towards the α1, 3-mannose branch, but a great promiscuity towards the α1, 6-mannose branch. Accordingly, a chemoenzymatic strategy was developed for the efficient synthesis of core-fucosylated asymmetric N -glycans.