Acetylpyrene-labelled 7-methylguanine nucleotides: unusual fluorescence properties and application to decapping scavenger activity monitoring. Issue 16 (15th March 2016)

• Record Type: Journal Article
• Authors: Kasprzyk, Renata; Kowalska, Joanna; Wieczorek, Zbigniew; Szabelski, Mariusz; Stolarski, Ryszard; Jemielity, Jacek
• Is Part Of: Organic & biomolecular chemistry. Volume 14:Issue 16(2016)
• Page Start: 3863
• Page End: 3868
• Publication Date: 2016-03-15
• Subjects: Chemistry, Organic -- Periodicals; Bioorganic chemistry -- Periodicals; Chemistry, Physical organic -- Periodicals; 547
• DOI: 10.1039/c6ob00419a
• Languages: English
• ISSNs: 1477-0520

Abstract

Abstract : We describe acetylpyrene-labeled mRNA cap analogues with untypical fluorescent properties as probes for inhibitor screening of DcpS. Abstract : 7-Methylguanosine (m 7 G) nucleotides labelled with acetylpyrene (AcPy) were synthesized as fluorescent mRNA 5′ end (cap) analogues. The unique fluorescent properties of m 7 G–AcPy conjugates, different from G–AcPy, can be applied to studying various mRNA cap-related processes including the evaluation of putative inhibitors of DcpS enzyme—a therapeutic target in neuromuscular diseases.