Mass spectrometry-based monitoring of millisecond protein–ligand binding dynamics using an automated microfluidic platform. Issue 9 (24th March 2016)

• Record Type: Journal Article
• Authors: Cong, Yongzheng; Katipamula, Shanta; Trader, Cameron D.; Orton, Daniel J.; Geng, Tao; Baker, Erin S.; Kelly, Ryan T.
• Is Part Of: Lab on a chip. Volume 16:Issue 9(2016)
• Page Start: 1544
• Page End: 1548
• Publication Date: 2016-03-24
• Subjects: Miniature electronic equipment -- Periodicals; Combinatorial chemistry -- Periodicals; Biotechnology -- Periodicals; 543.0813
• DOI: 10.1039/c6lc00183a
• Languages: English
• ISSNs: 1473-0197

Abstract

Abstract : We present a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and an integrated electrospray ionization source for mass spectrometry-based monitoring of protein–ligand binding dynamics. Abstract : Characterizing protein–ligand binding dynamics is crucial for understanding protein function and for developing new therapeutic agents. We present a novel microfluidic platform that features rapid mixing of protein and ligand solutions, variable incubation times, and an integrated electrospray ionization source for mass spectrometry-based monitoring of protein–ligand binding dynamics. This platform offers many advantages, including solution-based binding, label-free detection, automated operation, rapid mixing, and low sample consumption.