Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s. Issue 36 (14th April 2016)

• Record Type: Journal Article
• Authors: Weissenborn, Martin J.; Notonier, Sandra; Lang, Sarah-Luise; Otte, Konrad B.; Herter, Susanne; Turner, Nicholas J.; Flitsch, Sabine L.; Hauer, Bernhard
• Is Part Of: Chemical communications. Volume 52:Issue 36(2016)
• Page Start: 6158
• Page End: 6161
• Publication Date: 2016-04-14
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc01749e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A whole cell screening assay for terminal hydroxylases was developed based on a readily available galactose oxidase enzyme. Abstract : A readily available galactose oxidase (GOase) variant was used to develop a whole cell screening assay. This endpoint detection system was applied in a proof-of-concept approach by screening a focussed mutant library. This led to the discovery of the thus far most active P450 Marinobacter aquaeolei mutant catalysing the terminal hydroxylation of fatty acids.