Nonspecific protein labeling of photoaffinity linkers correlates with their molecular shapes in living cells. Issue 34 (4th April 2016)

• Record Type: Journal Article
• Authors: Park, Hankum; Koo, Ja Young; Srikanth, Yellamelli V. V.; Oh, Sangmi; Lee, Jiyoon; Park, Jongmin; Park, Seung Bum
• Is Part Of: Chemical communications. Volume 52:Issue 34(2016)
• Page Start: 5828
• Page End: 5831
• Publication Date: 2016-04-04
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc01426g
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The molecular shape of photoaffinity linkers significantly influences their nonspecific protein labeling of the cellular proteome. Abstract : Herein we report molecular shape-dependent nonspecific labeling of photoaffinity linkers (PLs) in the cellular proteome. Linear PLs have a greater tendency to engage in nonspecific binding than branched PLs. Exploiting this property, we discovered a smaller branched diazirine-based PL as the best photoaffinity probe with minimal nonspecific binding characteristics from among 5 probes with different PLs.