A simple method for enhancing the bioorthogonality of cyclooctyne reagent12. Issue 31 (24th March 2016)
- Record Type:
- Journal Article
- Title:
- A simple method for enhancing the bioorthogonality of cyclooctyne reagent12. Issue 31 (24th March 2016)
- Main Title:
- A simple method for enhancing the bioorthogonality of cyclooctyne reagent12
- Authors:
- Tian, He
Sakmar, Thomas P.
Huber, Thomas - Abstract:
- Abstract : The cross-reactivity between some cyclooctynes and thiols limits the bioorthogonality of the strain-promoted azide–alkyne cycloaddition reaction. Abstract : The cross-reactivity between some cyclooctynes and thiols limits the bioorthogonality of the strain-promoted azide–alkyne cycloaddition reaction. We show that a low concentration of β-mercaptoethanol significantly reduces the undesirable side reaction between bicyclononyne (BCN) and cysteine and while preserving free cysteines. We site-specifically label a genetically-encoded azido group in the visual photoreceptor rhodopsin to demonstrate the utility of the strategy.
- Is Part Of:
- Chemical communications. Volume 52:Issue 31(2016)
- Journal:
- Chemical communications
- Issue:
- Volume 52:Issue 31(2016)
- Issue Display:
- Volume 52, Issue 31 (2016)
- Year:
- 2016
- Volume:
- 52
- Issue:
- 31
- Issue Sort Value:
- 2016-0052-0031-0000
- Page Start:
- 5451
- Page End:
- 5454
- Publication Date:
- 2016-03-24
- Subjects:
- Chemistry -- Periodicals
540 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cc ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c6cc01321j ↗
- Languages:
- English
- ISSNs:
- 1359-7345
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3139.350000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 545.xml