Developments in spontaneous and coherent Raman scattering microscopic imaging for biomedical applications. (26th October 2015)

• Record Type: Journal Article
• Authors: Krafft, C.; Schie, I. W.; Meyer, T.; Schmitt, M.; Popp, J.
• Is Part Of: Chemical Society reviews. Volume 45:Number 7(2016:Apr.)
• Page Start: 1819
• Page End: 1849
• Publication Date: 2015-10-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cs00564g
• Languages: English
• ISSNs: 0306-0012

Abstract

Abstract : An overview about the instrumentation for spontaneous and coherent Raman scattering microscopic imaging is given with a focus on recent developments. Abstract : First, the potential role of Raman-based techniques in biomedicine is introduced. Second, an overview about the instrumentation for spontaneous and coherent Raman scattering microscopic imaging is given with a focus of recent developments. Third, imaging strategies are summarized including sequential registration with laser scanning microscopes, line imaging and global or wide-field imaging. Finally, examples of biomedical applications are presented in the context of single cells, laser tweezers, tissue sections, biopsies and whole animals.